Description of the test
Specifications: Titration of antibodies neutralising influenza virus in MDCK cell culture.
Result: The highest dilution conferring 50% neutralisation in cell culture is referred to as the neutralising titer. Seroconversion is defined as a 4-fold increase in titer between acute and convalescent serum.
Purpose of the test
Titration of neutralising antibodies against the influenzavirus using the seroneutralisation test.
Criteria for conducting this test under the reference activities
- Tests conducted within specific scientific studies
- Influenza syndrome after influenza vaccination (possible case of “vaccine failure”)
- Note: please call the NRC before requesting this test
Instructions for samples
Serum (coupled, before and after vaccination)
Serum (coupled, before and after exposure)
Minimal quantity required for analysis: 1 ml
Conservation and transport: refrigerated (2-8°C) or frozen (-20°C)
Instructions for transport
Transport at ambient temperature.
Inacceptible analysis requests
No contact with the NRC before submitting the analysis request.
If there are no clinical of virological indications of vaccine failure.
Turn around time
Normal duration of the analysis (TAT): 3 working days
Maximum duration: 4 weeks
Frequency of the analysis: once per 3 weeks
Reporting of test results
To decide at the start of the study