Detection of antibodies against Influenzavirus

Last updated on 2-2-2024 by Amber Van Laer

Description of the test

Specifications: Titration of antibodies neutralising influenza virus in MDCK cell culture.

Result: The highest dilution conferring 50% neutralisation in cell culture is referred to as the neutralising titer. Seroconversion is defined as a 4-fold increase in titer between acute and convalescent serum.

Purpose of the test 

Titration of neutralising antibodies against the influenzavirus using the seroneutralisation test.

Criteria for conducting this test under the reference activities 

  • Tests conducted within specific scientific studies
  • Influenza syndrome after influenza vaccination (possible case of “vaccine failure”)
  • ​Note: please call the NRC before requesting this test

Instructions for samples 

Sample type:

Serum (coupled, before and after vaccination)

Serum (coupled, before and after exposure)

Minimal quantity required for analysis: 1 ml

Conservation and transport: refrigerated (2-8°C) or frozen (-20°C)

Instructions for transport 

Transport at ambient temperature.

Inacceptible analysis requests

No contact with the NRC before submitting the analysis request.
If there are no clinical of virological indications of vaccine failure.​

Turn around time 

Normal duration of the analysis (TAT): 3 working days     

Maximum duration: 4 weeks

Frequency of the analysis: once per 3 weeks

Reporting of test results

To decide at the start of the study

Pathogen info

Influenza virus


Is the analysis accredited?

Materials and methods

Method reference: 

Turnaround time and time slots

Turnaround time: 
3 days

Service in charge of the analysis

Contact email

Analysis categories


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