Determination lineage Influenza B by real Time PCR

Last updated on 27-6-2024 by Amber Van Laer

Description of the test

RT-qPCR to determine the Influenza B lineage (Yamagata or Victoria).

Purpose of the test 

Determination of the lineage (Yamagata or Victoria) of Influenza B.  

Criteria for conducting this test under the reference activities 

Confirmation of a positive or undetermined result for influenza.

First line diagnosis in case of:

  • Severe acute respiratory infection (SARI) with ARDSECMO or death​
  • Influenza syndrome after influenza vaccination (possible case of “vaccine failure”)
  • Suspicion of antiviral resistance
  • Influenza syndrome upon return from abroad 
  • Surveillance

Instructions for samples

Sample types: 

  • nasopharyngeal swab
  • bronchial aspirate
  • broncho-alveolar lavage liquid.

Use a sterile tube with VTM (virus transport medium).

Minimal quantity: 500 µl.


Samples should be stored refrigerated (2-8°C, if <24h) before shipment. 

In case of shipment > 24h, store at -20°C or -70°C.

Instructions for transport​

Samples stored at 2-8°C can be transported at room temperature.

Samples stored at -20°C or -70°C should be transported on dry ice.

Transport time should be as short as possible (avoid transport during weekends and official holidays). ​

Transport samples using a hermetically closed recipient, triple packaging.

Inacceptable requests

The test analysis will not be performed in case of:

  • Insufficient or inappropriate sample
  • Inappropriate storage or transport
  • Uncompleted request form
  • Request not compliant with the criteria ​

Samples are stored during a maximum period of 1 year for other testing (re-test), if sufficient sample is available.

Reporting of test results

A report is sent by LIMS using e-mail, fax or regular post.

Pathogen info

Influenza virus


Is the analysis accredited?

Materials and methods

Method reference: 
SOP 13/3/31/F

Turnaround time and time slots

Turnaround time: 
15 days

Service in charge of the analysis

Contact email

Analysis categories


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