Further characterisation of B. pertussis isolates

Test description

• Genotyping of the virulence genes tcfA, ptxA, ptxC, ptxP and prn by whole genome sequencing.
• The polymorphism of the tcfA, ptxA and ptxC genes is studied by molecular techniques to determine an MLST type. In addition, the polymorphism of the pertactin gene is also examined.
• Antibiotic susceptibility
• Expression of pertactin and fim⅔.

A screening is performed for macrolide resistance.

Purpose of the test

As part of the surveillance of vaccination policy effectiveness and antibiotic prophylaxis, Bordetella pertussis is retrospectively studied in batches for virulence factors and antibiotic susceptibility.

Criteria for conducting this test as part of reference activities

All Bordetella pertussis isolates are examined for antibiotic susceptibility and pertactin and fim⅔ expression, and a representative number of isolates for genotyping and polymorphism.

Instructions for samples

N/A

Instructions for transport

N/A

Unacceptable applications

N/A

Turnaround time (and frequency of analysis)

These tests are carried out in batches on an annual basis.

Reporting of test results

• Results are reported in an overall report.
• Sentinel laboratories can obtain individual results upon request.