HEV genotyping (Sciensano)

Please fill in entirely the request form from the NRC for all analysis requests. You can download it below.

Pathogen

Hepatitis E Virus (HEV)

Assay description

LOINC Code : 60430-6

Details : 

Sanger sequencing performed from an in house nested PCR.

The protocol used is based on the one used at RIVM.

Results : 

Genotype & subtype.

A weak viral load often lead to sequencing failure.

The obtained sequence is aligned against a public database in order to determine the genotype and the subtype.

Accreditation : 

Non accredited assay.

Aim of the assay 

Gentyping of HEV by nested PCR and Sanger sequencing.

Criteria

Positive HEV PCR.

The requesting lab must mention the results of the PCR on the request form.

HEV genotyping is automatically performed in case of positive HEV PCR performed in our laboratory. Reporting is only done if the requesting lab has explicitly requested it via the request form.

Instructions related to sample

Type : Serum

Conservatory and shipment conditions : 

Conservation at 4°C before shipment.

Shipment at ambient temperature as soon as possible in a hermetic three layer package.

Requested quantities :

Preferred : 1 ml.

Minimal requirements : 0,5 ml.

Rejected request

Sample with insufficient volume.

Turnaround time

TAT : 30 opening days

Reporting

An analysis report can be sent by regular mail, e-mail, fax ou eHealth Box for each analysis request if the analysis request has been clearly specified.