HAV detection by PCR on saliva (Sciensano)

Please fill in entirely the request form from the NRC for all analysis requests. You can download it below.

Pathogen

Hepatitis A Virus (HAV)

Assay description

LOINC Code : 97523-5

Details : 

PCR in house.

The protocol used is based on https://doi.org/10.1016/j.jcv.2014.06.027 (Kodani et al. 2014. Rapid and sensitive approach to simultaneous detection of genomes of hepatitis A, B, C, D and E viruses, Journal of Clinical Virology, 61 (2): 260-264).

Results : 

Qualitative (positive/negative/undetermined).

In case of an undetermined result, the collection and the shipment of a second sample is recommended.

Cq value is given for information purposes. It does not give any information on the viral load. The more a Cq value is high, the less the viral load is high and vice-versa.

Accreditation : 

Non accredited assay.

Aim of the assay 

HAV RNA detection by PCR.

Diagnosis of an active HAV infection in a patient presenting with symptoms of hepatitis.

Criteria

Patient with symptoms of hepatitis and/or abnormal lab values.

The requesting lab must mention the clinical details suggestive of an infectious hepatitis on the request form.

Instructions related to sample

Type : 

Saliva

Oracol® sampling kit is available for 10 €. The request has to be made by e-mail to LMM@sciensano.be.

Conservatory and shipment conditions : 

Conservation at 4°C before shipment.

Shipment at ambient temperature as soon as possible in a hermetic three layer package.

Requested quantities :

Please consult the instructions provided with Oracol® sampling kit.

Rejected request

Sample with insufficient volume.

Turnaround time

TAT : 7 opening days

Reporting

An analysis report can be sent by regular mail, e-mail, fax ou eHealth Box for each analysis request.