HDV detection by PCR (Sciensano)

Last updated on 17-7-2023 by Amber Van Laer

Please fill in entirely the request form from the NRC for all analysis requests. You can download it below.



Hepatitis D Virus (HDV)

Assay description

LOINC Codes :  

  • 7906-1
  • 85512-2
  • 85513-0

Details : 

Assay perfomed with a commercial PCR kit : RealStar HDV RT-PCR 1.0 (Altona Diagnostics).

Results : 

Quantitative (IU/mL and log IU/mL).

The result of the quantification is only reported if the viral load is comprised between the limits of quantification defined by the standard curve (429 IU/mL – 429 000 IU/mL).

If the viral load is lower than the lower limit of quantification or above the upper limit of quantification that are defined by the standard curve, the result of the quantification will be given for information purpose only.

The limit of detection of our assay is of 13.4 IU/mL.

Accreditation : 

Accredited assay.

ISO 15189 | Quality and competence in medical laboratories

Aim of the assay 

HDV RNA detection by real time quantitative PCR.

Diagnosis of a HDV infection.


The patient must be positive for HDV serology and/or Hepatitis B Virus surface antigen (HBs Ag) and/or Hepatitis B Virus DNA (HBV PCR). The requesting lab must mention the clinical details suggestive of an infectious hepatitis on the request form.

HDV PCR is automatically performed in case of positive/undetermined HDV-IgTot performed in our laboratory. Reporting is done once the analysis is finished.

Instructions related to sample

Type : Serum

Conservatory and shipment conditions : 

Conservation at 4°C before shipment.

Shipment at ambient temperature as soon as possible in a hermetic three layer package.

Requested quantities :

Preferred : 1 ml.

Minimal requirements : 0,5 ml.

Rejected request

Sample with insufficient volume.

No positive results for HDV serology and/or HBs Ag and/or HBV PCR performed prior to the request and for which results are not mentioned on the request form.

Turnaround time

TAT : 7 opening days


An analysis report can be sent by regular mail, e-mail, fax ou eHealth Box for each analysis request.


Pathogen info

Hepatitis D virus


Is the analysis accredited?

Materials and methods

Method reference: 

Turnaround time and time slots

Turnaround time: 
7 days

Service in charge of the analysis

Contact person(s)

Contact email

Analysis categories


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