Measles: Genotyping of measles virus

Result

• Genotype A-B-C-D-E-F-G-H
• This test is not accredited.

Purpose of the test

Genotyping of Measles virus via end-point PCR and Sanger sequencing.

Criteria for conducting this test under the reference activities

Performed only on samples that are PCR-positive.

Unacceptable demands

If the genotype is already known for at least 5 cases per cluster or outbreak and there are no epidemiological or clinical indications for infection with a new or unusual genotype.

Instructions for samples

• Type:
  • Saliva (via Oracol® collection device, can be ordered from the NRC via LMM@sciensano.be)
  • Nasopharyngeal swab (dry swab, swab in UTM or swab in liquid Amiens medium)
• Sample collection: up to 7 days after rash apperance. Virus is detectable from 4 days before the onset of the rash until 7 days after the appearance of the rash (infectious period).
• Storage conditions: Store samples at 4°C before shipment
• Minimum volume: 0.5 ml

Instructions for transportation

Ship in a triple-wrapped package at room temperature.

Turn around time

30 business days

Communication of test results

The result can be communicated via mail, fax, email and eHealth.

A positive result will also be reported to your region’s health inspector.