Description of the test
Sequencing of influenza A and B (hemaglutinine & neuraminidase).
Purpose of the test
Genotypical characterisation of the hemaglutinine (HA) and neuraminidase (NA) gene of influenza A and B viruses by sequencing.
The objective is to detect mutations that are associated to differences in pathogenicity and resistance to antivirals.
Criteria for conducting this test under the reference activities
Confirmation of a positive or undetermined result for influenza.
First line diagnosis in case of:
- Severe acute respiratory infection (SARI) with ARDS, ECMO or death
- Influenza syndrome after influenza vaccination (possible case of “vaccine failure”)
- Suspicion of antiviral resistance
- Influenza syndrome upon return from abroad
- Surveillance
Instructions for samples
Sample types :
- nasopharyngeal swab
- bronchial aspirate
- broncho-alveolar lavage liquid.
Use a sterile tube with VTM (virus transport medium).
Minimal quantity: 500 µl.
Storage:
Samples should be stored refrigerated (2-8°C, if <24h) before shipment.
In case of shipment > 24h, store at -20°C or -70°C.
Instructions for transport
Samples stored at 2-8°C can be transported at room temperature.
Samples stored at -20°C or -70°C should be transported on dry ice.
Transport time should be as short as possible (avoid transport during weekends and official holidays).
Transport samples using an hermetically closed recipient, triple packaging.
Inacceptable requests
The test analysis will not be performed in case of:
- Insufficient or inappropriate sample
- Inappropriate storage or transport
- Uncompleted request form
- Request not compliant with the criteria
Samples are stored during a maximum period of 1 year for other testing (re-test), if sufficient sample is available.
Reporting of test results
A report is sent by LIMS using e-mail, fax or regular post.