Last updated on 20-1-2020 by Steven Van Gucht
Public Access
Published
Keywords
Abstract:
OBJECTIVE: The re-emergence and spreading of tropical viruses to new areas raised worldwide a wave of concern. To treat patients in an early stage and prevent diffusion of the outbreak, an early diagnosis, and thus fast and adequate detection, is needed. To this aim, a multiplex reverse transcription real-time polymerase chain reaction TaqMan® method was designed to detect universally Zika and chikungunya viruses.
DESIGN: Two methods, targeting different genome segments, were selected from literature for each virus, adapted for high genome coverage and joined into a four-plex assay which…