Application of a Commercial Real-Time RT-PCR Assay for Surveillance of Classical Swine Fever: Evaluation by Testing Sequential Tissue and Blood Samples

Last updated on 14-1-2022 by Andy Haegeman

Public Access


Peer reviewed scientific article




The applicability of the commercial TaqVet CSFV real-time RT-PCR assay (TaqVet CSF) as a diagnostic tool for herd surveillance of classical swine fever (CSF) was evaluated in an experimental setup on a group of 28 pigs infected with a moderately virulent classical swine fever virus (CSFV) strain of wild boar origin (‘11722-WIL’). Based on the viral distribution determined in tissue by the real-time RT-PCR assay (rRT-PCR), the tonsils were found the most suitable tissue for CSFV detection. In the tonsils, the viral genome was detected during the incubation phase, as early as 2 days post infe…

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