Detection and characterization of verotoxigenic Escherichia coli by a VTEC/EHEC multiplex PCR in porcine faeces and pig carcass swabs36718

Last updated on 22-8-2019 by Anonymous (not verified)

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Abstract:

To determine whether pigs and pig carcasses are a reservoir of verotoxigenic-producing Escherichia coli, a sensitive and rapid isolation method was developed. A VTEC/EHEC multiplex PCR method was developed to test all samples taken at the farm (n=289) and at the slaughterhouse (n=233). From the 137 positive VTEC/EHEC multiplex PCR farm samples, 54 strains were isolated that carried one virulence gene. Of these, thirty-one strains carried the vt2e variant of the vt2 gene, which causes oedemic disease in young pigs, four strains contained the hlyA gene and 19 the eaeA gene. All strains isolat…

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