Development of a dot-blot assay for the detection of mould-specific IgE in the Belgian population

Last updated on 22-8-2019 by Anonymous (not verified)

Peer reviewed scientific article




Background Data on mould sensitization in the general population are scarce and mostly on Aspergillus fumigatus, Alternaria alternata and Cladosporium herbarum. Objectives To validate a dot-blot assay for the detection of specific IgE and evaluate the prevalence of mould sensitization in a healthy population. Methods The dot-blot assay was validated against the CAP test. Sensitization rate to ten common indoor and outdoor mould species in 344 serum samples was calculated. For each serum with more than one reactivity, the “major sensitization” define…

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