Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study.

Last updated on 22-8-2019 by Anonymous (not verified)

Peer reviewed scientific article

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Abstract:

BACKGROUND: Indoor air pollution caused by fungal contamination is suspected to have a public health impact. Monitoring of the composition of the indoor airborne fungal contaminants is therefore important. To avoid problems linked to culture-dependent protocols, molecular methods are increasingly being proposed as an alternative. Among these molecular methods, the polymerase chain reaction (PCR) and the real-time PCR are the most frequently used tools for indoor fungal detection. However, even if these tools have demonstrated their appropriate performance, some of them are not able to discr…

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