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Evaluation of the Premi Test Salmonella, a commercial low-density DNA microarray system intended for routine identification and typing of Salmonella enterica.

Last updated on 22-8-2019 by Anonymous (not verified)
Animal health  

Peer reviewed scientific article

SCIENSANO

Authors

P Wattiau; Weijers, Thijs; Andreoli, Peter; Schliker, Christine; Veken, Heidi Vander; Maas, Henny M E; Verbruggen, Anjo J; Heck, Max E O C; Wannet, Wim J; Hein Imberechts; Vos, Pieter

Keywords

  1. Bacterial Typing Techniques
  2. Consumer Product Safety
  3. DNA, Bacterial
  4. Food Contamination
  5. Food Microbiology
  6. Gene Expression Profiling
  7. Genotype
  8. Humans
  9. Oligonucleotide Array Sequence Analysis
  10. Reproducibility of Results
  11. Salmonella enterica
  12. Sensitivity and Specificity
  13. Serotyping
  14. Species Specificity

Abstract:

A new commercial system based on genetic profiling and aimed at identifying Salmonella enterica serovars was evaluated by comparing its performance with classical serotyping on 443 strains. Within 62 serovars represented, 60 gave unique genetic profiles while 2 were undistinguishable. Results were obtained within 8 h, were reproducible and clear-cut. The system allowed single-tube processing of the samples and required no peculiar technical skill. It showed interesting potential for routine laboratory testing.
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Associated health topics:

Animal health

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