TY - JOUR T1 - Comparison of eae, tir, espA and espB genes of bovine and human attaching and effacing Escherichia coli by multiplex polymerase chain reaction44 JF - FEMS Microbiol.Lett. Y1 - 1999 A1 - Bernard China A1 - Goffaux,F. A1 - Pirson,V. A1 - J.G. Mainil KW - 0 KW - a KW - Adhesins,Bacterial KW - Animals KW - article KW - Bacterial Outer Membrane Proteins KW - Bacterial Proteins KW - Bacterial Typing Techniques KW - Belgium KW - Carrier Proteins KW - Cattle KW - Cell KW - classification KW - Comparison KW - Diarrhea KW - e KW - EHEC KW - Escherichia coli KW - Escherichia coli Proteins KW - Faculty KW - gene KW - Genes KW - Genes,Bacterial KW - genetics KW - Healthy KW - Human KW - Humans KW - im KW - IS KW - journal KW - Laboratories KW - Medicine KW - Membrane Proteins KW - methods KW - Order KW - pathogenicity KW - physiology KW - polymerase chain reaction KW - present KW - Print KW - proportion KW - protein KW - Proteins KW - Receptor KW - receptors KW - Receptors,Cell Surface KW - region KW - Research KW - Research Support KW - SB - IM KW - strain KW - Universities KW - university KW - veterinary KW - Veterinary Medicine KW - virulence AB - Attaching and effacing Escherichia coli (AEEC) virulence genes include the eae, the tir, the espA and the espB genes. These genes have been sequenced from several AEEC strains. The sequences alignments revealed the presence of constant and variable regions. Multiplex polymerase chain reactions were developed, in order to determine the subtype of each gene present in a particular isolate. AEEC strains isolated from calves dead of diarrhea, from healthy calves and from infected humans were compared. The same pathotypes were found in sick and healthy calves but in inverted proportion. These pathotypes were also found in human AEEC. Although, the human EHEC strains from serotype O157 possessed their own pathotype VL - 178 CP - 1 U1 - 38561 M3 - S0378-1097(99)00352-3 [pii] ER -