TY - JOUR T1 - Unusual Aspergillus species in patients with cystic fibrosis36887 JF - Med.Mycol. Y1 - 2010 A1 - Symoens,F. A1 - Haase,G. A1 - Pihet,M. A1 - Carrere,J. A1 - H. Beguin A1 - Degand,N. A1 - Mely,L. A1 - Bouchara,J.P. KW - 0 KW - a KW - ADOLESCENT KW - Agent KW - Agents KW - ALL KW - Amphotericin B KW - analysi KW - analysis KW - Antifungal Agents KW - article KW - AS KW - Aspergillus KW - at KW - Belgium KW - Brussels KW - Case KW - case reports KW - chronic KW - classification KW - culture KW - Culture Media KW - Cystic Fibrosis KW - Dna KW - drug effects KW - electronic KW - European KW - Eurotiales KW - Fibrosis KW - Follow up KW - FOLLOW-UP KW - Follow-Up Studies KW - genetics KW - growth KW - health KW - hospital KW - hospitals KW - Humans KW - identification KW - im KW - Institute KW - IS KW - isolation & purification KW - IT KW - journal KW - LEVEL KW - Lung Diseases,Fungal KW - Male KW - MEDIA KW - Microbial Sensitivity Tests KW - microbiology KW - Mycological Typing Techniques KW - n KW - Observation KW - Patient KW - patients KW - pharmacology KW - public KW - public health KW - Public-health KW - Pulmonary Aspergillosis KW - report KW - reports KW - routine KW - Sample KW - Samples KW - SB - IM KW - Sequence Analysis KW - Sequence Analysis,DNA KW - Species Specificity KW - sputum KW - study KW - Temperature AB - Poorly sporulating Aspergillus isolates from patients with cystic fibrosis (CF) are generally identified in routine procedures as Aspergillus spp. In this study, we identified and characterized 11 isolates belonging to two unusual Aspergillus species of the section Fumigati (A. lentulus and Neosartorya pseudofischeri) recovered from four different patients. Aspergillus lentulus was found occasionally during a 10-year follow-up study of one CF patient colonized by A. fumigatus. Neosartorya pseudofischeri was isolated from three patients followed in different European hospitals. This species was recovered from two sputum samples of one patient, and from four successive samples of the two other patients, suggesting that it may be responsible for chronic colonization. Both species were isolated together with A. fumigatus. Isolates from both species did not grow at 50 degrees C, and DNA sequence analysis, together with further morphological observations permitted identification at the species level. Growth at different temperatures and antifungal susceptibility were also investigated. All the isolates of N. pseudofischeri exhibited a very low susceptibility to voriconazole (VRZ) whereas a very low susceptibility to VRZ and amphotericin B was seen with the A. lentulus isolates VL - 48 Suppl 1 U1 - 38376 M3 - http://dx.doi.org/10.3109/13693786.2010.501345 ER -