TY - JOUR T1 - An integrated strategy combining DNA walking and NGS to detect GMO JF - Food Chemistry Y1 - 2017 A1 - Marie-Alice Fraiture A1 - Philippe Herman A1 - N. Papazova A1 - De Loose, Marc A1 - Deforce, Dieter A1 - Ruttink, Tom A1 - Nancy Roosens KW - detection KW - DNA walking KW - GMO KW - Next-generation sequencing KW - qPCR AB -

Recently, we developed a DNA walking system for the detection and characterization of a broad spectrum of GMOs in routine analysis of food/feed matrices. Here, we present a new version with improved throughput and sensitivity by coupling the DNA walking system to Pacific Bioscience® Next-generation sequencing technology. The performance of the new strategy was thoroughly assessed through several assays. First, we tested its detection and identification capability on grains with high or low GMO content. Second, the potential impacts of food processing were investigated using rice noodle samples. Finally, GMO mixtures and a real-life sample were analyzed to illustrate the applicability of the proposed strategy in routine GMO analysis. In all tested samples, the presence of multiple GMOs was unambiguously proven by the characterization of transgene flanking regions and the combinations of elements that are typical for transgene constructs.

VL - 232 M3 - https://doi.org/10.1016/j.foodchem.2017.03.067 ER -