TY - JOUR T1 - Validation of a sensitive DNA walking strategy to characterise unauthorised GMOs using model food matrices mimicking common rice products JF - Food Chemistry Y1 - 2015 A1 - Marie-Alice Fraiture A1 - Philippe Herman A1 - Taverniers, Isabel A1 - De Loose, Marc A1 - Van Nieuwerburgh, Filip A1 - Deforce, Dieter A1 - Nancy Roosens KW - detection KW - DNA walking KW - Rice food KW - SENSITIVITY KW - Transgene flanking region KW - Unauthorised GMO AB -

To identify unauthorised GMOs in food and feed matrices, an integrated approach has recently been developed targeting pCAMBIA family vectors, highly present in transgenic plants. Their presence is first assessed by qPCR screening and is subsequently confirmed by characterising the transgene flanking regions, using DNA walking. Here, the DNA walking performance has been thoroughly tested for the first time, regarding the targeted DNA quality and quantity. Several assays, on model food matrices mimicking common rice products, have allowed to determine the limit of detection as well as the potential effects of food mixture and processing. This detection system allows the identification of transgenic insertions as low as 10 HGEs and was not affected by the presence of untargeted DNA. Moreover, despite the clear impact of food processing on DNA quality, this method was able to cope with degraded DNA. Given its specificity, sensitivity, reliability, applicability and practicability, the proposed approach is a key detection tool, easily implementable in enforcement laboratories.

VL - 173 M3 - https://doi.org/10.1016/j.foodchem.2014.09.148 ER -