TY - JOUR T1 - Immunogenicity and protective efficacy of a tuberculosis DNA vaccine. JF - Nat Med Y1 - 1996 A1 - Huygen, K A1 - Content, J A1 - Denis, O A1 - Montgomery, D L A1 - Yawman, A M A1 - Deck, R R A1 - C M DeWitt A1 - Orme, I M A1 - Baldwin, S A1 - D'Souza, C A1 - Drowart, A A1 - Lozes, E A1 - Vandenbussche, P A1 - Van Vooren, J P A1 - Liu, M A A1 - Ulmer, J B KW - Animals KW - Antibodies, Bacterial KW - Antibody Formation KW - Antigens, Bacterial KW - BCG Vaccine KW - Cytokines KW - Disease Models, Animal KW - DNA, Bacterial KW - Immunity, Cellular KW - mice KW - Mice, Inbred BALB C KW - Mice, Inbred C57BL KW - Mycobacterium tuberculosis KW - T-Lymphocytes KW - Tuberculosis KW - Tumor Cells, Cultured AB -

Tuberculosis is the most widespread and lethal infectious disease affecting humans. Immunization of mice with plasmid DNA constructs encoding one of the secreted components of Mycobacterium tuberculosis, antigen 85 (Ag85), induced substantial humoral and cell-mediated immune responses and conferred significant protection against challenge with live M. tuberculosis and M. bovis bacille Calmette-Guérin (BCG). These results indicate that immunization with DNA encoding a mycobacterial antigen provides an efficient and simple method for generating protective immunity and that this technique may be useful for defining the protective antigens of M. tuberculosis, leading to the development of a more effective vaccine.

VL - 2 CP - 8 U1 - https://www.ncbi.nlm.nih.gov/pubmed/8705859?dopt=Abstract M3 - 10.1038/nm0896-893 ER -