TY - JOUR T1 - Inter-laboratory evaluation of the performance parameters of a Lateral Flow Test device for the detection of Bluetongue virus-specific antibodies. JF - J Virol Methods Y1 - 2016 A1 - Jean-Baptiste Hanon A1 - Valerie Vandenberge A1 - Deruelle, Matthias A1 - Ilse De Leeuw A1 - Kris De Clercq A1 - Steven Van Borm A1 - F. Koenen A1 - Liu, Lihong A1 - Hoffmann, Bernd A1 - Batten, Carrie Anne A1 - Zientara, Stéphan A1 - Bréard, Emmanuel A1 - Yves Van der Stede KW - Animals KW - Antibodies, Viral KW - Bluetongue KW - Bluetongue virus KW - Cattle KW - Cattle Diseases KW - Chromatography, Affinity KW - Cooperative Behavior KW - Cross Reactions KW - Enzyme-Linked Immunosorbent Assay KW - Hemorrhagic Disease Virus, Epizootic KW - Laboratory Proficiency Testing KW - Reproducibility of Results KW - Ruminants KW - Sensitivity and Specificity KW - Serologic Tests KW - Sheep AB -
Bluetongue (BT) is a viral vector-borne disease affecting domestic and wild ruminants worldwide. In this study, a commercial rapid immuno-chromatographic method or Lateral Flow Test (LFT) device, for the detection of BT virus-specific antibodies in animal serum, was evaluated in an international inter-laboratory proficiency test. The evaluation was done with sera samples of variable background (ruminant species, serotype, field samples, experimental infections, vaccinated animals). The diagnostic sensitivity was 100% (95% C.I. [90.5-100]) and the diagnostic specificity was 95.2% (95% C.I. [76.2-99.9]). The repeatability (accordance) and reproducibility (concordance) were 100% for seropositive samples but were lower for two of the seronegative samples (45% and 89% respectively). The analytical sensitivity, evaluated by testing positive sera at increasing dilutions was better for the BT LFT compared to some commercial ELISAs. Seroconversion of an infected sheep was detected at 4 days post infection. Analytical specificity was impaired by cross-reactions observed with some of the samples seropositive for Epizootic Haemorrhagic Disease Virus (EHDV). The agreement (Cohen's kappa) between the LFT and a commercial BT competitive ELISA was 0.79 (95% CI [0.62-0.95]). Based on these results, it can be concluded that the BT LFT device is a rapid and sensitive first-line serological test that can be used in the field, especially in areas endemic for the disease where there is a lack of diagnostic facilities.
VL - 228 U1 - https://www.ncbi.nlm.nih.gov/pubmed/26687976?dopt=Abstract M3 - 10.1016/j.jviromet.2015.12.001 ER -