TY - JOUR T1 - 2'-C-methylcytidine as a potent and selective inhibitor of the replication of foot-and-mouth disease virus. JF - Antiviral Res Y1 - 2007 A1 - Goris, Nesya A1 - De Palma, Armando A1 - Toussaint, Jean-François A1 - Musch, Ina A1 - Neyts, Johan A1 - Kris De Clercq KW - Animals KW - Antiviral Agents KW - Cricetinae KW - Cytidine KW - Enterovirus B, Human KW - Foot-and-Mouth Disease KW - Foot-and-Mouth Disease Virus KW - Reverse Transcriptase Polymerase Chain Reaction KW - RNA, Viral KW - Swine KW - Swine Vesicular Disease KW - Virus Replication AB -

We report on the potent and selective in vitro antiviral activity of 2'-C-methylcytidine (2'-C-MetCyt) against foot-and-mouth disease virus (FMDV). FMDV belongs to the Picornaviridae and has the potential to cause devastating epidemics in livestock. The 50% and 90% effective concentrations (EC50 and EC90) for inhibition of the FMDV-induced cytopathic effect (CPE) formation were 6.4+/-3.8 and 10.8+/-5.4 microM. Comparable EC50 values for inhibition of viral RNA synthesis were observed. Treatment of FMDV-infected BHK-21 cells with 77 microM 2'-C-MetCyt resulted in a (1.6-3.2)x10(3)-fold reduction of infectious virus yield. Time-of-drug addition experiments suggest that 2'-C-MetCyt interacts with viral replication at a time point that coincides with the onset of intracellular viral RNA synthesis. In contrast to emergency vaccination, a potent and selective antiviral agent may provide almost immediate (prophylactic/therapeutic) protection against infection and thus constitute an important alternative/supplementary option to contain outbreaks such as those caused by FMDV.

VL - 73 CP - 3 U1 - http://www.ncbi.nlm.nih.gov/pubmed/17055073?dopt=Abstract M3 - 10.1016/j.antiviral.2006.09.007 ER -