TY - JOUR T1 - Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies. JF - PLoS One Y1 - 2013 A1 - Bréard, Emmanuel A1 - Lara, Estelle A1 - Comtet, Loïc A1 - Viarouge, Cyril A1 - Doceul, Virginie A1 - Desprat, Alexandra A1 - Vitour, Damien A1 - Pozzi, Nathalie A1 - Ann Brigitte Cay A1 - Nick De Regge A1 - Pourquier, Philippe A1 - Schirrmeier, Horst A1 - Hoffmann, Bernd A1 - Beer, Martin A1 - Sailleau, Corinne A1 - Zientara, Stéphan KW - Animals KW - Antibodies, Neutralizing KW - Antibodies, Viral KW - Bunyaviridae Infections KW - Cattle KW - Enzyme-Linked Immunosorbent Assay KW - Europe KW - Fluorescent Antibody Technique, Indirect KW - Gene Expression KW - Neutralization Tests KW - Nucleocapsid Proteins KW - Orthobunyavirus KW - Reagent Kits, Diagnostic KW - Recombinant Proteins KW - Reproducibility of Results KW - Roc Curve KW - Sheep AB -

A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derived from sheep, goat and bovine sera collected in France and Germany (n = 1515) in 2011 and 2012 were categorized according to the results of a virus neutralization test (VNT) or an indirect immuno-fluorescence assay (IFA). The specificity was evaluated with 1364 sera from sheep, goat and bovine collected in France and Belgium before 2009. Overall agreement between VNT and ELISA was 98.9% and 98.3% between VNT and IFA, indicating a very good concordance between the different techniques. Although cross-reactions with other Orthobunyavirus from the Simbu serogroup viruses might occur, it is a highly sensitive, specific and robust ELISA-test validated to detect anti-SBV antibodies. This test can be applied for SBV sero-diagnostics and disease-surveillance studies in ruminant species in Europe.

VL - 8 CP - 1 U1 - http://www.ncbi.nlm.nih.gov/pubmed/23335964?dopt=Abstract M3 - 10.1371/journal.pone.0053446 ER -