TY - JOUR T1 - Measurement of systemic and local respiratory cell-mediated immunity after influenza infection in chickens. JF - Vet Immunol Immunopathol Y1 - 2011 A1 - Fabienne Rauw A1 - Anbari, Sofia A1 - Thierry van den Berg A1 - Bénédicte Lambrecht KW - Animals KW - Antigens, Viral KW - Chickens KW - Immunity, Cellular KW - Immunity, Mucosal KW - In Vitro Techniques KW - Influenza A Virus, H5N2 Subtype KW - Influenza in Birds KW - Interferons KW - Lung KW - Lymphocyte Activation KW - Spleen KW - T-Lymphocytes KW - Trachea AB -

The detection of ChIFN production after ex vivo antigenic-stimulation of T-lymphocytes has been evaluated for the first time, as a tool to assess cell-mediated immunity (CMI) after avian influenza (AI) infection in 10-day-old SPF chickens. Preliminarily, recall antigens have been produced either by concentrating and inactivating the whole virus or by dissociating the viral proteins. Biologically and structurally intact forms of the viral proteins were isolated by non-ionic detergents while heating, chemical agents and ionic detergent used for virus inactivation altered the antigenic viral components. The n-octyl-B-D-gluco-pyranoside treatment at low temperature was very efficient to produce AI antigenic proteins used for evaluation of ChIFN production after ex vivo antigenic-stimulation of splenic and peripheral lymphocytes. In addition, protocols to isolate lymphocytes from the respiratory tract - the trachea and the lung - have been adapted for local CMI evaluation after similar ex vivo recall assay. Specific AI CMI in the spleen, the blood and the lung was detected for 5 weeks after low pathogenic AI (LPAI) infection in chickens, while further development is needed for tracheal CMI measurement.

VL - 143 CP - 1-2 U1 - http://www.ncbi.nlm.nih.gov/pubmed/21696832?dopt=Abstract M3 - 10.1016/j.vetimm.2011.05.029 ER -