TY - JOUR T1 - Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology. JF - Vaccine Y1 - 2008 A1 - Letellier, Carine A1 - Boxus, Mathieu A1 - Rosar, Laurent A1 - Toussaint, Jean-François A1 - Walravens, Karl A1 - S. Roels A1 - Meyer, Gilles A1 - Letesson, Jean-Jacques A1 - Pierre Kerkhofs KW - Animals KW - Bronchoalveolar Lavage Fluid KW - Cattle KW - Cattle Diseases KW - CD8-Positive T-Lymphocytes KW - Cell Proliferation KW - Immunization, Secondary KW - Interferon-gamma KW - Lung KW - Nucleocapsid Proteins KW - Plasmids KW - Pneumonia KW - Respiratory Syncytial Virus Infections KW - Respiratory Syncytial Virus Vaccines KW - Respiratory Syncytial Virus, Bovine KW - Vaccines, DNA KW - Vaccines, Subunit AB -

Respiratory syncytial virus (RSV) is a major cause of respiratory disease in both cattle and young children. Despite the development of vaccines against bovine (B)RSV, incomplete protection and exacerbation of subsequent RSV disease have occurred. In order to circumvent these problems, calves were vaccinated with the nucleocapsid protein, known to be a major target of CD8(+) T cells in cattle. This was performed according to a DNA prime-protein boost strategy. The results showed that DNA vaccination primed a specific T-cell-mediated response, as indicated by both a lymphoproliferative response and IFN-gamma production. These responses were enhanced after protein boost. After challenge, mock-vaccinated calves displayed gross pneumonic lesions and viral replication in the lungs. In contrast, calves vaccinated by successive administrations of plasmid DNA and protein exhibited protection against the development of pneumonic lesions and the viral replication in the BAL fluids and the lungs. The protection correlated to the cell-mediated immunity and not to the antibody response.

VL - 26 CP - 37 U1 - http://www.ncbi.nlm.nih.gov/pubmed/18644416?dopt=Abstract M3 - 10.1016/j.vaccine.2008.06.100 ER -