TY - JOUR T1 - A rapid and environmental friendly determination of the dithiocarbamate metabolites ethylenethiourea and propylenethiourea in fruit and vegetables by ultra high performance liquid chromatography tandem mass spectrometry. JF - J Chromatogr A Y1 - 2011 A1 - Bonnechère, Aurore A1 - Hanot, Vincent A1 - Joris Van Loco KW - Chromatography, High Pressure Liquid KW - Drug Stability KW - Ethylenethiourea KW - Fruit KW - Fungicides, Industrial KW - Linear Models KW - Pesticide residues KW - Reproducibility of Results KW - Sensitivity and Specificity KW - Tandem Mass Spectrometry KW - Thiocarbamates KW - Thiourea KW - Vegetables AB -

Previous published methods for the analysis of ETU and PTU are time-consuming and furthermore use dichloromethane (DCM) for extraction or clean-up. This study details the development and validation of a rapid method that combines a simple extraction step with UHPLC-ESI(+)-MS/MS. This is the first application of UHPLC-MS/MS to analyse these compounds. Besides that, we replaced DCM with a more environmental-friendly solvent. The analytical performance was evaluated with the analysis of spiked celery samples at 50 μg kg(-1) (LOQ) and 300 μg kg(-1). The recoveries were between 65% and 90% for ETU and between 71% and 127% for PTU with RSDs in repeatability and reproducibility conditions below 10% for ETU. This method is rapid (a chromatographic run time of 2 min) and can easily be performed (no laborious clean-up). The presented method is environmental friendly with significant reduction in solvent consumption.

VL - 1218 CP - 29 U1 - http://www.ncbi.nlm.nih.gov/pubmed/21676400?dopt=Abstract M3 - 10.1016/j.chroma.2011.04.083 ER -