TY - JOUR T1 - Multi locus variable-number tandem repeat (MLVA) typing tools improved the surveillance of Salmonella enteritidis: a 6 years retrospective study. JF - PLoS One Y1 - 2015 A1 - Sophie Bertrand A1 - De Lamine de Bex, Guillaume A1 - Wildemauwe, Christa A1 - Lunguya, Octavie A1 - Phoba, Marie France A1 - Ley, Benedikt A1 - Jacobs, Jan A1 - Vanhoof, Raymond A1 - Wesley Mattheus KW - Anti-Bacterial Agents KW - Bacterial Typing Techniques KW - Disease Outbreaks KW - Genetic Loci KW - Humans KW - Minisatellite Repeats KW - Public Health Surveillance KW - Retrospective Studies KW - Salmonella enteritidis KW - Salmonella Infections AB -

Surveillance of Salmonella enterica subsp. enterica serovar Enteritidis is generally considered to benefit from molecular techniques like multiple-locus variable-number of tandem repeats analysis (MLVA), which allow early detection and confinement of outbreaks. Here, a surveillance study, including phage typing, antimicrobial susceptibility testing and MLVA on 1,535 S. Enteritidis isolates collected between 2007 and 2012, was used to evaluate the added value of MLVA for public health surveillance in Belgium. Phage types PT4, PT8, PT21, PT1, PT6, PT14b, PT28 and PT13 dominate the Belgian S. Enteritidis population. The isolates of S. Enteritidis were most frequently susceptible to all antibiotics tested. 172 different MLVA profiles were detected, of which 9 frequent profiles included 67.2% of the S. Enteritidis population. During a serial passage experiment on selected isolates to investigate the in vitro stability of the 5 MLVA loci, no variations over time were observed indicating that the MLVA profiles were stable. The MLVA profile of isolates originating from different outbreaks in the Democratic Republic of the Congo (DRC) between 2010 and 2011 were distinct from any of the MLVA profiles found in Belgian isolates throughout the six year observational period and demonstrates that MLVA improves public health surveillance of S. Enteritidis. However, MLVA should be complemented with other subtyping methods when investigating outbreaks is caused by the most common MLVA profile.

VL - 10 CP - 2 U1 - http://www.ncbi.nlm.nih.gov/pubmed/25693200?dopt=Abstract M3 - 10.1371/journal.pone.0117950 ER -