TY - JOUR T1 - Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO. JF - Sci Rep Y1 - 2018 A1 - Marie-Alice Fraiture A1 - Assia Saltykova A1 - Sigrid C.J. De Keersmaecker A1 - Nancy Roosens ED - Hoffmann,S. ED - Raf Winand ED - Deforce, Dieter ED - Kevin Vanneste KW - DNA walking KW - food and feed safety KW - GMO KW - GMO detection KW - method KW - method development KW - nanopore sequencing KW - Unauthorised GMO AB -

In order to strengthen the current genetically modified organism (GMO) detection system for unauthorized GMO, we have recently developed a new workflow based on DNA walking to amplify unknown sequences surrounding a known DNA region. This DNA walking is performed on transgenic elements, commonly found in GMO, that were earlier detected by real-time PCR (qPCR) screening. Previously, we have demonstrated the ability of this approach to detect unauthorized GMO via the identification of unique transgene flanking regions and the unnatural associations of elements from the transgenic cassette. In the present study, we investigate the feasibility to integrate the described workflow with the MinION Next-Generation-Sequencing (NGS). The MinION sequencing platform can provide long read-lengths and deal with heterogenic DNA libraries, allowing for rapid and efficient delivery of sequences of interest. In addition, the ability of this NGS platform to characterize unauthorized and unknown GMO without any a priori knowledge has been assessed.

VL - 8 CP - 1 M3 - 10.1038/s41598-018-26259-x ER -