%0 Journal Article %J Eur J Immunol %D 2000 %T Distinct VH repertoires in primary and secondary B cell lymphocyte subsets in the preimmune repertoire of A/J mice: the CRI-A idiotype is preferentially associated with the HSA(low) B cell subset. %A C Masungi Luko %A Vansanten, G %A Ryelandt, M %A Denis, O %A Wuilmart, C %A Andris, F %A Van Acker, A %A Brait, M %A Cloquet, J P %A Ismaili, N %A Nisol, F %A Latinne, D %A Brown, A %A Leo, O %A Bazin, H %A Urbain, J %K Animals %K Antigens %K B-Lymphocyte Subsets %K Base Sequence %K Gene Rearrangement %K Hemocyanins %K Immunoglobulin D %K Immunoglobulin Heavy Chains %K Immunoglobulin Idiotypes %K Immunoglobulin M %K Immunoglobulin Variable Region %K Immunologic Memory %K mice %K Mice, Inbred BALB C %K Molecular Sequence Data %X

The anti-arsonate immune response of A/J mice is characterized by the occurrence of several recurrent idiotypes with a different temporal pattern of expression. The CRI-A idiotype is typically a memory idiotype since it appears late in the primary and dominates the secondary as well as subsequent immune responses. The CRI-C idiotype is present throughout the responses, including the primary one. Naive adult A/J mice treated repeatedly with anti-mu or anti-delta monoclonal antibodies exhibit a completely different balance of HSA(low) and HSA(high) B cell subsets and an opposite idiotype profile after immunization with p-azophenylarsonate coupled to hemocyanin. Anti-mu treatment leads to a striking enhancement of the HSA(low) cell subset associated with an earlier important synthesis of CRI-A(+) antibodies, while anti-delta treatment enhances significantly the HSA(high) compartment with a strong decrease of CRI-A and persistence of CRI-C1 antibodies. Semiquantitative PCR analysis reveals that the presence of CRI-A transcripts is associated with the HSA(low) compartment, while CRI-C transcripts are mainly associated with HSA(high) B cell subsets. This has been demonstrated with spleen cells of adult A/J mice treated with anti-mu or anti-delta antibodies and also with purified B cell subsets of unimmunized adult A/J mice and on neonatal spleen cells. It appears that the memory (CRI-A) idiotype is selected into the HSA(low) B cell subset before antigen arrival.

%B Eur J Immunol %V 30 %P 2312-22 %8 2000 Aug %G eng %N 8 %1 https://www.ncbi.nlm.nih.gov/pubmed/10940922?dopt=Abstract %& 2312 %R 10.1002/1521-4141(2000)30:8<2312::AID-IMMU2312>3.0.CO;2-P