%0 Journal Article %J J Virol Methods %D 2016 %T Inter-laboratory evaluation of the performance parameters of a Lateral Flow Test device for the detection of Bluetongue virus-specific antibodies. %A Jean-Baptiste Hanon %A Valerie Vandenberge %A Deruelle, Matthias %A Ilse De Leeuw %A Kris De Clercq %A Steven Van Borm %A F. Koenen %A Liu, Lihong %A Hoffmann, Bernd %A Batten, Carrie Anne %A Zientara, Stéphan %A Bréard, Emmanuel %A Yves Van der Stede %K Animals %K Antibodies, Viral %K Bluetongue %K Bluetongue virus %K Cattle %K Cattle Diseases %K Chromatography, Affinity %K Cooperative Behavior %K Cross Reactions %K Enzyme-Linked Immunosorbent Assay %K Hemorrhagic Disease Virus, Epizootic %K Laboratory Proficiency Testing %K Reproducibility of Results %K Ruminants %K Sensitivity and Specificity %K Serologic Tests %K Sheep %X

Bluetongue (BT) is a viral vector-borne disease affecting domestic and wild ruminants worldwide. In this study, a commercial rapid immuno-chromatographic method or Lateral Flow Test (LFT) device, for the detection of BT virus-specific antibodies in animal serum, was evaluated in an international inter-laboratory proficiency test. The evaluation was done with sera samples of variable background (ruminant species, serotype, field samples, experimental infections, vaccinated animals). The diagnostic sensitivity was 100% (95% C.I. [90.5-100]) and the diagnostic specificity was 95.2% (95% C.I. [76.2-99.9]). The repeatability (accordance) and reproducibility (concordance) were 100% for seropositive samples but were lower for two of the seronegative samples (45% and 89% respectively). The analytical sensitivity, evaluated by testing positive sera at increasing dilutions was better for the BT LFT compared to some commercial ELISAs. Seroconversion of an infected sheep was detected at 4 days post infection. Analytical specificity was impaired by cross-reactions observed with some of the samples seropositive for Epizootic Haemorrhagic Disease Virus (EHDV). The agreement (Cohen's kappa) between the LFT and a commercial BT competitive ELISA was 0.79 (95% CI [0.62-0.95]). Based on these results, it can be concluded that the BT LFT device is a rapid and sensitive first-line serological test that can be used in the field, especially in areas endemic for the disease where there is a lack of diagnostic facilities.

%B J Virol Methods %V 228 %P 140-50 %8 2016 Feb %G eng %1 https://www.ncbi.nlm.nih.gov/pubmed/26687976?dopt=Abstract %R 10.1016/j.jviromet.2015.12.001