%0 Journal Article %J Transbound Emerg Dis %D 2015 %T Full-Genome Sequencing of Four Bluetongue Virus Serotype 11 Viruses. %A Frank Vandenbussche %A Sailleau, C %A Rosseel, T %A Desprat, A %A Viarouge, C %A Richardson, J %A Eschbaumer, M %A Hoffmann, B %A Kris De Clercq %A Bréard, E %A Zientara, S %K Animals %K Base Sequence %K Bluetongue %K Bluetongue virus %K Europe %K Genome, Viral %K Molecular Sequence Data %K Phylogeny %K Serogroup %K Sheep %K Vaccination %K Viral Vaccines %X

Recently, a contamination incident was described in which the challenge inoculum used in a bluetongue virus serotype 8 (BTV-8) vaccination trial was contaminated with a BTV-11 virus that was closely related to the Belgian BTV-11 virus from 2008. This study reports the first complete genome sequences of four BTV-11 viruses: the BTV-11 contaminant, BTV-11 reference strain, BTV-11 vaccine strain and a recently isolated BTV-11 field strain from Martinique. Full-genome analysis showed that these viruses belong to serotype 11/nucleotype A and cluster together with other western topotype bluetongue viruses. Detailed comparisons of the genomes further indicated that the contaminant was derived from the BTV-11 reference strain, as they were distinguished by a single synonymous nucleotide substitution. The previously reported partial sequence of genome segment 2 of the Belgian BTV-11 was found to be identical to that of the BTV-11 vaccine strain, indicating that it most likely was the BTV-11 vaccine strain. These findings also suggest that the BTV-11 contaminant and the Belgian BTV-11 are not the same viruses. Finally, comparison of the reference and vaccine strain did not allow determining the amino acid substitutions that contribute to the attenuated phenotype.

%B Transbound Emerg Dis %V 62 %P 565-71 %8 2015 Oct %G eng %N 5 %1 https://www.ncbi.nlm.nih.gov/pubmed/24750582?dopt=Abstract %R 10.1111/tbed.12178