%0 Journal Article %J Antiviral Res %D 2007 %T 2'-C-methylcytidine as a potent and selective inhibitor of the replication of foot-and-mouth disease virus. %A Goris, Nesya %A De Palma, Armando %A Toussaint, Jean-François %A Musch, Ina %A Neyts, Johan %A Kris De Clercq %K Animals %K Antiviral Agents %K Cricetinae %K Cytidine %K Enterovirus B, Human %K Foot-and-Mouth Disease %K Foot-and-Mouth Disease Virus %K Reverse Transcriptase Polymerase Chain Reaction %K RNA, Viral %K Swine %K Swine Vesicular Disease %K Virus Replication %X

We report on the potent and selective in vitro antiviral activity of 2'-C-methylcytidine (2'-C-MetCyt) against foot-and-mouth disease virus (FMDV). FMDV belongs to the Picornaviridae and has the potential to cause devastating epidemics in livestock. The 50% and 90% effective concentrations (EC50 and EC90) for inhibition of the FMDV-induced cytopathic effect (CPE) formation were 6.4+/-3.8 and 10.8+/-5.4 microM. Comparable EC50 values for inhibition of viral RNA synthesis were observed. Treatment of FMDV-infected BHK-21 cells with 77 microM 2'-C-MetCyt resulted in a (1.6-3.2)x10(3)-fold reduction of infectious virus yield. Time-of-drug addition experiments suggest that 2'-C-MetCyt interacts with viral replication at a time point that coincides with the onset of intracellular viral RNA synthesis. In contrast to emergency vaccination, a potent and selective antiviral agent may provide almost immediate (prophylactic/therapeutic) protection against infection and thus constitute an important alternative/supplementary option to contain outbreaks such as those caused by FMDV.

%B Antiviral Res %V 73 %P 161-8 %8 2007 Mar %G eng %N 3 %1 http://www.ncbi.nlm.nih.gov/pubmed/17055073?dopt=Abstract %R 10.1016/j.antiviral.2006.09.007