%0 Journal Article %J Mucosal Immunol %D 2015 %T CCR2(+)CD103(-) intestinal dendritic cells develop from DC-committed precursors and induce interleukin-17 production by T cells. %A Scott, C L %A Bain, C C %A Wright, P B %A Sichien, D %A Kotarsky, K %A Persson, E K %A Luda, K %A Guilliams, M %A Bénédicte Lambrecht %A Agace, W W %A Milling, S Wf %A Mowat, A M %K Animals %K Antigens, CD %K Cell Differentiation %K Dendritic Cells %K Humans %K Immunophenotyping %K Integrin alpha Chains %K Interferon Regulatory Factors %K Interleukin-12 %K Interleukin-17 %K Intestinal Mucosa %K Macrophages %K mice %K Mice, Transgenic %K Monocytes %K Phagocytes %K Phenotype %K Receptors, CCR2 %K T-Lymphocyte Subsets %K Th17 Cells %X

The identification of intestinal macrophages (mφs) and dendritic cells (DCs) is a matter of intense debate. Although CD103(+) mononuclear phagocytes (MPs) appear to be genuine DCs, the nature and origins of CD103(-) MPs remain controversial. We show here that intestinal CD103(-)CD11b(+) MPs can be separated clearly into DCs and mφs based on phenotype, gene profile, and kinetics. CD64(-)CD103(-)CD11b(+) MPs are classical DCs, being derived from Flt3 ligand-dependent, DC-committed precursors, not Ly6C(hi) monocytes. Surprisingly, a significant proportion of these CD103(-)CD11b(+) DCs express CCR2 and there is a selective decrease in CD103(-)CD11b(+) DCs in mice lacking this chemokine receptor. CCR2(+)CD103(-) DCs are present in both the murine and human intestine, drive interleukin (IL)-17a production by T cells in vitro, and show constitutive expression of IL-12/IL-23p40. These data highlight the heterogeneity of intestinal DCs and reveal a bona fide population of CCR2(+) DCs that is involved in priming mucosal T helper type 17 (Th17) responses.

%B Mucosal Immunol %V 8 %P 327-39 %8 2015 Mar %G eng %N 2 %1 http://www.ncbi.nlm.nih.gov/pubmed/25138666?dopt=Abstract %R 10.1038/mi.2014.70