%0 Journal Article %J J Chromatogr A %D 2011 %T A rapid and environmental friendly determination of the dithiocarbamate metabolites ethylenethiourea and propylenethiourea in fruit and vegetables by ultra high performance liquid chromatography tandem mass spectrometry. %A Bonnechère, Aurore %A Hanot, Vincent %A Joris Van Loco %K Chromatography, High Pressure Liquid %K Drug Stability %K Ethylenethiourea %K Fruit %K Fungicides, Industrial %K Linear Models %K Pesticide residues %K Reproducibility of Results %K Sensitivity and Specificity %K Tandem Mass Spectrometry %K Thiocarbamates %K Thiourea %K Vegetables %X

Previous published methods for the analysis of ETU and PTU are time-consuming and furthermore use dichloromethane (DCM) for extraction or clean-up. This study details the development and validation of a rapid method that combines a simple extraction step with UHPLC-ESI(+)-MS/MS. This is the first application of UHPLC-MS/MS to analyse these compounds. Besides that, we replaced DCM with a more environmental-friendly solvent. The analytical performance was evaluated with the analysis of spiked celery samples at 50 μg kg(-1) (LOQ) and 300 μg kg(-1). The recoveries were between 65% and 90% for ETU and between 71% and 127% for PTU with RSDs in repeatability and reproducibility conditions below 10% for ETU. This method is rapid (a chromatographic run time of 2 min) and can easily be performed (no laborious clean-up). The presented method is environmental friendly with significant reduction in solvent consumption.

%B J Chromatogr A %V 1218 %P 4627-31 %8 2011 Jul 22 %G eng %N 29 %1 http://www.ncbi.nlm.nih.gov/pubmed/21676400?dopt=Abstract %R 10.1016/j.chroma.2011.04.083