%0 Journal Article %J Appl Microbiol Biotechnol %D 2019 %T Development of a real-time PCR method for the genoserotyping of Salmonella Paratyphi B variant Java. %A Mathieu Gand %A Wesley Mattheus %A Assia Saltykova %A Nancy Roosens %A Katelijne Dierick %A Marchal, Kathleen %A Sigrid C.J. De Keersmaecker %A Sophie Bertrand %X

Discriminating between D-tartrate fermenting and non-fermenting strains of Salmonella enterica subsp. enterica serotype Paratyphi B is of major importance as these two variants have different pathogenic profiles. While D-tartrate non-fermenting S. Paratyphi B isolates are the causative agent of typhoid-like fever, D-tartrate fermenting isolates (also called variant Java) of the same serotype trigger the less dangerous gastroenteritis. The determination of S. Paratyphi B variants requires a time-consuming process and complex biochemical tests. Therefore, a quadruplex real-time PCR method, based on the allelic discrimination of molecular markers selected from the scientific literature and from whole genome sequencing data produced in-house, was developed in this study, to be applied to Salmonella isolates. This method was validated with the analysis of 178 S. Paratyphi B (D-tartrate fermenting and non-fermenting) and other serotypes reaching an accuracy, compared with the classical methods, of 98% for serotyping by slide agglutination and 100% for replacement of the biochemical test. The developed real-time PCR permits to save time and to obtain an accurate identification of a S. Paratyphi B serotype and its D-tartrate fermenting profile, which is needed in routine laboratories for fast and efficient diagnostics.

%B Appl Microbiol Biotechnol %V 103 %8 2019 Jun %G eng %N 12 %R 10.1007/s00253-019-09854-4