<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Wuyts,V.</style></author><author><style face="normal" font="default" size="100%">Wesley Mattheus</style></author><author><style face="normal" font="default" size="100%">Nancy Roosens</style></author><author><style face="normal" font="default" size="100%">Marchal,K.</style></author><author><style face="normal" font="default" size="100%">Sophie Bertrand</style></author><author><style face="normal" font="default" size="100%">Sigrid C.J. De Keersmaecker</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium</style></title><secondary-title><style face="normal" font="default" size="100%">Appl Microbiol Biotechnol</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Antibiotic</style></keyword><keyword><style  face="normal" font="default" size="100%">Antibiotic resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">AS</style></keyword><keyword><style  face="normal" font="default" size="100%">cause</style></keyword><keyword><style  face="normal" font="default" size="100%">causes</style></keyword><keyword><style  face="normal" font="default" size="100%">Concordance</style></keyword><keyword><style  face="normal" font="default" size="100%">data</style></keyword><keyword><style  face="normal" font="default" size="100%">detection</style></keyword><keyword><style  face="normal" font="default" size="100%">EPIDEMIOLOGICAL</style></keyword><keyword><style  face="normal" font="default" size="100%">gene</style></keyword><keyword><style  face="normal" font="default" size="100%">Genes</style></keyword><keyword><style  face="normal" font="default" size="100%">genomic</style></keyword><keyword><style  face="normal" font="default" size="100%">INFECTION</style></keyword><keyword><style  face="normal" font="default" size="100%">infections</style></keyword><keyword><style  face="normal" font="default" size="100%">International</style></keyword><keyword><style  face="normal" font="default" size="100%">investigation</style></keyword><keyword><style  face="normal" font="default" size="100%">IS</style></keyword><keyword><style  face="normal" font="default" size="100%">IT</style></keyword><keyword><style  face="normal" font="default" size="100%">Laboratories</style></keyword><keyword><style  face="normal" font="default" size="100%">LEVEL</style></keyword><keyword><style  face="normal" font="default" size="100%">Luminex</style></keyword><keyword><style  face="normal" font="default" size="100%">Marker</style></keyword><keyword><style  face="normal" font="default" size="100%">Markers</style></keyword><keyword><style  face="normal" font="default" size="100%">method</style></keyword><keyword><style  face="normal" font="default" size="100%">microsphere array</style></keyword><keyword><style  face="normal" font="default" size="100%">MOL-PCR</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular</style></keyword><keyword><style  face="normal" font="default" size="100%">molecular markers</style></keyword><keyword><style  face="normal" font="default" size="100%">Network</style></keyword><keyword><style  face="normal" font="default" size="100%">Objective</style></keyword><keyword><style  face="normal" font="default" size="100%">observed</style></keyword><keyword><style  face="normal" font="default" size="100%">ON</style></keyword><keyword><style  face="normal" font="default" size="100%">outbreak</style></keyword><keyword><style  face="normal" font="default" size="100%">outbreaks</style></keyword><keyword><style  face="normal" font="default" size="100%">resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">result</style></keyword><keyword><style  face="normal" font="default" size="100%">results</style></keyword><keyword><style  face="normal" font="default" size="100%">S</style></keyword><keyword><style  face="normal" font="default" size="100%">Salmonella</style></keyword><keyword><style  face="normal" font="default" size="100%">Salmonella enterica</style></keyword><keyword><style  face="normal" font="default" size="100%">Salmonella typhimurium</style></keyword><keyword><style  face="normal" font="default" size="100%">stability</style></keyword><keyword><style  face="normal" font="default" size="100%">Subtyping</style></keyword><keyword><style  face="normal" font="default" size="100%">Surveillance</style></keyword><keyword><style  face="normal" font="default" size="100%">Technique</style></keyword><keyword><style  face="normal" font="default" size="100%">Type</style></keyword><keyword><style  face="normal" font="default" size="100%">use</style></keyword><keyword><style  face="normal" font="default" size="100%">VALIDATION</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2015</style></year><pub-dates><date><style  face="normal" font="default" size="100%">0/10/2015</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">8149</style></number><volume><style face="normal" font="default" size="100%">99</style></volume><pages><style face="normal" font="default" size="100%">8137 - 8149</style></pages><isbn><style face="normal" font="default" size="100%">0175-7598</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Subtyping below the serovar level is essential for surveillance and outbreak detection and investigation of Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) and its monophasic variant 1,4,[5],12:i:- (S. 1,4,[5],12:i:-), frequent causes of foodborne infections. In an attempt to overcome the intrinsic shortcomings of currently used subtyping techniques, a multiplex oligonucleotide ligation-PCR (MOL-PCR) assay was developed which combines different types of molecular markers in a high-throughput microsphere suspension array. The 52 molecular markers include prophage genes, amplified fragment length polymorphism (AFLP) elements, Salmonella genomic island 1 (SGI1), allantoinase gene allB, MLVA locus STTR10, antibiotic resistance genes, single nucleotide polymorphisms (SNPs) and phase 2 flagellar gene fljB. The in vitro stability of these markers was confirmed in a serial passage experiment. The validation of the MOL-PCR assay for subtyping of S. Typhimurium and S. 1,4,[5],12:i:- on 519 isolates shows that the method is rapid, reproducible, flexible, accessible, easy to use and relatively inexpensive. Additionally, a 100 % typeability and a discriminatory power equivalent to that of phage typing were observed, and epidemiological concordance was assessed on isolates of 2 different outbreaks. Furthermore, a data analysis method is provided so that the MOL-PCR assay allows for objective, computerised data analysis and data interpretation of which the results can be easily exchanged between different laboratories in an international surveillance network.&lt;/p&gt;
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