<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Laurence Delbrassinne</style></author><author><style face="normal" font="default" size="100%">Mirjana Andjelkovic</style></author><author><style face="normal" font="default" size="100%">Rajkovic, Andreja</style></author><author><style face="normal" font="default" size="100%">N Botteldoorn</style></author><author><style face="normal" font="default" size="100%">Mahillon, Jacques</style></author><author><style face="normal" font="default" size="100%">Joris Van Loco</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Follow-up of the Bacillus cereus emetic toxin production in penne pasta under household conditions using liquid chromatography coupled with mass spectrometry.</style></title><secondary-title><style face="normal" font="default" size="100%">Food Microbiol</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Food Microbiol.</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bacillus cereus</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacterial Toxins</style></keyword><keyword><style  face="normal" font="default" size="100%">Chromatography, Liquid</style></keyword><keyword><style  face="normal" font="default" size="100%">Depsipeptides</style></keyword><keyword><style  face="normal" font="default" size="100%">Food Contamination</style></keyword><keyword><style  face="normal" font="default" size="100%">Food Handling</style></keyword><keyword><style  face="normal" font="default" size="100%">Food Microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Mass Spectrometry</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2011 Aug</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">28</style></volume><pages><style face="normal" font="default" size="100%">1105-9</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Two outbreak-related Bacillus cereus emetic strains were investigated for their growth and cereulide production potential in penne pasta at 4, 8 and 25 °C during 7-day storage. Cereulide production was detected and quantified by LC-MS method (LOD of 1 ng/ml, LOQ of 5 ng/ml) and growth was determined by culture-based enumeration. Inoculated B. cereus strains (10(5) CFU/g) were able to reach counts of more than 10(8) CFU/g and cereulide production of about 500 ng/g already after 3 days of storage at 25 °C. Interestingly, a constant increase of the toxin was noticed during incubation at ambient temperature storage: the cereulide was continuously produced during the bacterial stationary growth phase reaching maximal amounts at the end of the experiment (7 days, concentration of about 1000 ng/g). Strictly respected cold chain temperature as 4 °C did not allow any detectable cereulide production for any of the two tested strains. At the limited temperature abuse of 8 °C, a detectable amount of cereulide was observed after two days for one of the strain (TIAC303) (&lt;LOQ). These results confirm that cereulide production is controlled by multiple factors (from type of strain to temperature) and that prolonged storage time plays a crucial role for consumer safety.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/21569959?dopt=Abstract</style></custom1></record></records></xml>