<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Marie-Alice Fraiture</style></author><author><style face="normal" font="default" size="100%">Gabriella Ujhelyi</style></author><author><style face="normal" font="default" size="100%">Jaroslava Ovesna</style></author><author><style face="normal" font="default" size="100%">D. Van Geel</style></author><author><style face="normal" font="default" size="100%">Sigrid C.J. De Keersmaecker</style></author><author><style face="normal" font="default" size="100%">Assia Saltykova</style></author><author><style face="normal" font="default" size="100%">N. Papazova</style></author><author><style face="normal" font="default" size="100%">Nancy Roosens</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">MinION sequencing technology to characterize unauthorized GM petunia plants circulating on the European Union market</style></title><secondary-title><style face="normal" font="default" size="100%">Scientific reports</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">amplicon sequencing</style></keyword><keyword><style  face="normal" font="default" size="100%">detection</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA walking</style></keyword><keyword><style  face="normal" font="default" size="100%">GM petunia</style></keyword><keyword><style  face="normal" font="default" size="100%">Oxford Nanopore Technologies</style></keyword><keyword><style  face="normal" font="default" size="100%">Real-time PCR</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year></dates><volume><style face="normal" font="default" size="100%">9</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;In order to characterize unauthorized genetically modified petunia, an integrated strategy has been applied here on several suspected petunia samples from the European market. More precisely, DNA fragments of interest were produced by DNA walking anchored on key targets, earlier detected by real-time PCR screening analysis, to be subsequently sequenced using the MinION platform from Oxford Nanopore Technologies. This way, the presence of genetically modified petunia was demonstrated via the characterization of their transgene flanking regions as well as unnatural associations of elements from their transgenic cassette.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">7141</style></issue></record></records></xml>