<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pieter-Jan Ceyssens</style></author><author><style face="normal" font="default" size="100%">An Van den Bossche</style></author><author><style face="normal" font="default" size="100%">Lac Kim Phan</style></author><author><style face="normal" font="default" size="100%">Koenraad Van Hoorde</style></author><author><style face="normal" font="default" size="100%">Wesley Mattheus</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A molecular assay for rapidly distinguishing the AviPro SALMONELLA VAC T vaccine strain from wild-type field isolates</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Microbiological Methods</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Luminex-based multiplex test</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular differentiation</style></keyword><keyword><style  face="normal" font="default" size="100%">Salmonella typhimurium</style></keyword><keyword><style  face="normal" font="default" size="100%">Vac T</style></keyword><keyword><style  face="normal" font="default" size="100%">vaccin</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May-2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">184</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Rapid differentiation of the AviPro Salmonella VAC T strain from wild-type&amp;nbsp;Salmonella&amp;nbsp;ser. Typhimurium isolates is essential for the monitoring of veterinary isolates and targeted control actions. The distinction between the two strain types is routinely made by phenotypic&amp;nbsp;antimicrobial resistance&amp;nbsp;testing, but this sometime leads to ambiguous results with major economic implications. In this study, we used whole-genome sequencing to identify conserved and specific mutations in resistance and virulence genes which enable to distinguish field and vaccine strains. Based on this information, we developed and validated (n&amp;nbsp;=&amp;nbsp;199) a Luminex-based assay targeting seven specific single-nucleotide polymorphisms. This molecular test is able to distinguish both&amp;nbsp;Salmonella&amp;nbsp;ser. Typhimurium types with 100% sensitivity and specificity within one working day.&lt;/p&gt;
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