Fine quantification of avian influenza H5N1 escape mutant quasispecies populations using mutation-specific real-time PCR.

Last updated on 18-10-2022 by Anonymous (not verified)

Peer reviewed scientific article

SCIENSANO

Abstract:

Influenza viruses have a rapid replication cycle, using enzymes without proofreading capacity and generating multiple virus quasispecies during replication. The identification and quantification of these quasispecies populations require time-consuming and expensive cloning and sequencing approaches. In the present study, we developed mutation-specific real-time PCR (RT-PCR) tests for the fine quantification of mutations in a background of wild-type sequences. As a proof-of-concept model, we developed mutation-specific RT-PCR tests to quantify antibody escape mutations during passage under m…

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