Fluorene degradation by Sphingomonas sp. LB126 proceeds through protocatechuic acid: a genetic analysis.

Last updated on 22-8-2019 by Anonymous (not verified)

Peer reviewed scientific article

SCIENSANO

Abstract:

Sphingomonas sp. LB126 is able to utilize fluorene as sole source of carbon and energy. In the present study, a mutagenic vector was constructed and a “plasmid rescue” strategy was set up to isolate a 16.5-kb DNA fragment containing genes required for fluorene degradation. A 14.5-kb portion of the cloned DNA was sequenced revealing thirteen open reading frames. Two encoded hypothetical proteins (FldE and FldY) similar to transcriptional regulators and one (ORF360) located on an IS-like element (ISSsp126) encoded a putative transposase. Three other putative proteins (FldB, FldU and FldV) dis…

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