Molecular typing of Aspergillus fumigatus strains by sequence-specific DNA primer (SSDP) analysis36675

Last updated on 23-8-2019 by Anonymous (not verified)

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Peer reviewed scientific article



A PCR typing method has been developed and tested to investigate the polymorphism of clinical strains of Aspergillus fumigatus. Firstly, the DNA fragments from random amplified polymorphic DNA (RAPD) patterns of nine epidemiologically and geographically non-related monosporal strains of A. fumigatus were cloned and sequenced. The pairs of five sequence-specific DNA primers (SSDP), characteristic of the 5’ and 3’ extremities of the RAPD products, were then used in high stringency PCR to type 43 clinical strains of A. fumigatus from 13 patients, according to the presence or absence of a singl…

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