Opinion: Airtightness for Decontamination by Fumigation of High-Containment Laboratories

Last updated on 12-9-2019 by Fanny Coppens

Peer reviewed scientific article



Introduction: While the European legislation states that laboratories of high-containment must be sealable for fumigation, they do not prescribe a minimal value for airtightness. Starting from a previous study in which we measured the airtightness in 4 BSL-3 laboratories with blower-door tests, we discuss the connection between airtightness and a successful decontamination by fumigation. Methods: Biological indicators (BIs) consisting of spores of Geobacillus stearothermophilus on metal disks were laid out in laboratories of different levels of airtightness before performing a fumi…

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