Phage–Host Protein–Protein Interactions Using Strep-tag® II Purifications

Last updated on 23-8-2019 by Anonymous (not verified)

Public Access

Published

Scientific book or chapter

English

Abstract:

After injecting their genome into the bacterial host cell, bacteriophages need to convert the host metabolism toward efficient phage production. For this, specific proteins have evolved which interact with key host proteins to inhibit, activate or redirect the function of these proteins. Since 70% of the currently annotated phage genes are hypothetical proteins of unknown function, the identification and characterization of these phage proteins involved in host–phage protein–protein interactions remains challenging. Here, we describe a method to identify phage proteins involved in host–phag…

Associated health topics:

QR code

QR code for this page URL