Selection of quinolone resistance in Streptococcus pneumoniae exposed in vitro to subinhibitory drug concentrations

Last updated on 23-8-2019 by Anonymous (not verified)

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Peer reviewed scientific article


OBJECTIVES: Does exposure to subinhibitory concentrations of quinolones favour overexpression of efflux pumps or selection of target site mutations? METHODS: ATCC 49,619 (fully susceptible) and SP32 (clinical isolate with PmrA-mediated efflux and mutation in ParE) were exposed for 24 h in broth to ciprofloxacin, levofloxacin, moxifloxacin or garenoxacin at concentrations of 0.5x the MIC, with daily re-adjustments for up to 13 days. Efflux was detected phenotypically (decrease in MIC in the presence of reserpine), and expression of pmrA and patA/patB was measured by real-time PCR and compara…

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