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Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
laboratories in the future. The method was first tested on minced beef spiked with shiga toxin-producing E.coli (STEC) at very low level (5 CFU /25g). After enrichment for 16 or 24 hours in buffered peptone ...
Residential green space in association with the methylation status in a CpG site within the promoter region of the placental serotonin receptor HTR2A
from the Green Map of Flanders in seven buffers (50 m-3 km) and stratified into low (<3 m) and high (≥3 m) vegetation. Residential nature was calculated using the Land use Map of Flanders. We ...
Residing in urban areas with higher green space is associated with lower mortality risk: A census-based cohort study with ten years of follow-up
greenness (2006) [normalized difference vegetation index (NDVI) and modified soil-adjusted vegetation index (MSAVI2)] within buffers of 300 m, 500 m, and 1000 m; 2) surrounding green space (2006) [Urban Atlas ...
Environmental exposures and health behavior in association with mental health: a study design.
green space is defined in buffers of different size (50 m — 5000 m) using land cover data in ArcGIS 10 software. For the statistical analysis multivariate linear and logistic regressions as well as ...
Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates
relationships between isolates were not affected by the DNA extraction kit choice, irrespective of the presence of confounding factors such as EDTA in DNA solution buffers. Nevertheless, completeness of STEC ...
Residential green space, air pollution, socioeconomic deprivation and cardiovascular medication sales in Belgium: A nationwide ecological study
socioeconomic deprivation were used as predictors of CV medication sales and were estimated from the number of patches of forest, census tract relative forest cover and relative forest cover within a 600 m buffer ...
A simple dilute-and-shoot method for screening and simultaneous quantification of nicotine and alkaloid impurities in electronic cigarette refills (e-liquids) by UHPLC-DAD
excluded. In addition, small changes to the column temperature, pH and molar concentration of the mobile phase buffer were deliberately introduced in order to assess the robustness of the method. Only ...
Chromatographic fingerprinting as a strategy to identify regulated plants in illegal herbal supplements.
Tribulus terrestris. The full factorial design demonstrated that for all three plant references a C8 column (250mm×4.6mm; 5µm particle size) is best suited; methanol and an ammonium formate buffer (pH 3) ...
Application of LC-MS/MS MRM to Determine Staphylococcal Enterotoxins (SEB and SEA) in Milk.
quantify two types of SE (A and B) spiked in milk and buffer solution. SE extraction and concentration was performed according to the European Screening Method developed by the European Reference Laboratory ...
Evaluation of detection methods for non-O157 Shiga toxin-producing Escherichia coli from food.
in parallel in Buffered Peptone Water (BPW) and Brila broth. After enrichment, detection was performed using real-time PCR (qPCR), and isolation using two chromogenic agar media, CHROM agar™ STEC and ...