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Structural elucidation of a novel mechanism for the bacteriophage-based inhibition of the RNA degradosome.

The 2.2 Å crystal structure reveals that this novel homo-dimeric protein has no identifiable structural homologues. Our biochemical data indicate that acidic patches on the convex outer surface bind RN ...

Experimental approaches to identify small RNAs and their diverse roles in bacteria- what we have learnt in one decade of MicA research5238

function, (iii) structural analysis. The complex mechanisms of action of MicA deliver some original insights in the sRNA field which includes the existence of dimer formation or simultaneous cis and trans ...

HIV-1 IN/Pol recruits LEDGF/p75 into viral particles.

preintegration complex to the chromatin through a direct interaction with integrase (IN). Small molecules that bind the LEDGF /p75 binding pocket of the HIV IN dimer (LEDGIN s) block HIV replication through ...

Use of Strep-tag II for rapid detection and purification of Mycobacterium tuberculosis recombinant antigens secreted by Streptomyces lividans

dimer. The obtained results indicate that the Strep-tagII is proteolytically stable in Streptomyces and does not interfere with the membrane translocation of Ag85A and Rv2626c. A comparison of reactivity ...

Non-competitive interaction between raclopride and spiperone on human D-receptors in intact Chinese hamster ovary cells.

dissociation rate of [(3)H]-spiperone. The easiest way to interpret the present findings in molecular terms is to assume that D(2L)-receptors or their dimeric complexes possess two distinct binding sites: one ...

Drosophila molting neurohormone bursicon is a heterodimer and the natural agonist of the orphan receptor DLGR24402

Media Culture Media,Conditioned de Dimerization Drosophila Drosophila Proteins EN ET genetics Genome Honey hormonal Hormone hormones identify im Invertebrate Hormones IS journal MEDIA metabolism Molecular ...

Antimycobacterial activity of synthetic pamamycins

pamamycin-607 and pamamycin-621A and a non-naturally occurring cyclic dimer of pamamycin-607, i.e. yukomycin. RESULTS: Pamamycin-607 was the most active of the three macrocycles and was more active against M. ...

Crystal structure of a dimeric oxidized form of human peroxiredoxin 5.

Source: J Mol Biol, Volume 337, Issue 5 (2004) Keywords: Crystallization Crystallography, X-Ray Dimerization Disulfides Dithiothreitol Electrophoresis, Polyacrylamide Gel Humans Mass Spectrometry Models, ...

Identification of DNA sequences recognized by VirF, the transcriptional activator of the Yersinia yop regulon.

activator of the system. virF encodes a DNA-binding protein related to the AraC family of transcriptional activators. The VirF protein from Yersinia enterocolitica is a 30-kDa protein that forms dimers in ...

Viral interference of the bacterial RNA metabolism machinery.

this ‘degradosome interacting protein’ or Dip, to adopt an ‘open-claw’ dimeric structure that presents acidic patches on its outer surface which hijack 2 conserved RNA binding sites on the scaffold ...

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