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Development of a multiplex immunoassay for simultaneous detection of antigens in DTaP vaccines

that could be implemented for routine potency assessment of vaccines. Our work focused on the development of a Luminex-based multiplex assay (four to six-plex assay depending on the number of pertussis ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses

combined in a four-plex assay that was thoroughly validated in-house. The SCREENED tool was used to evaluate the sequence coverage of the method. Results The full validation approach showed that the new ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.

literature for each virus, adapted for high genome coverage and joined into a four-plex assay which was thoroughly in-house validated. The SCREENED tool was used to evaluate the sequence coverage of ...

RNA-based drug susceptibility testing of Mycobacterium tuberculosis

performed directly on crude cell lysate using a combination of the bead-based MagPixTM (Luminex) and QuantigeneTM Plex (Thermo Fischer) technology. Normalized, relative genes expression levels (control vs ...

Guidelines for optimisation of a multiplex oligonucleotide ligation-PCR for characterisation of microbial pathogens in a microsphere suspension array.

PCR assay. Therefore, we here describe our observations made during the optimisation of a 20-plex MOL- PCR assay for subtyping of Salmonella Typhimurium with the aim to provide a possible workflow as ...

Development of a Luminex®-based molecular alternative for classical microbiological subtyping techniques, Salmonella phage typing as a case study

pathogen Phage type plex assay present S Salmonella SCREENING SELECTED SOFTWARE study Subtyping Surveillance Target Technique Type typing Abstract: The PhD-project SalMolType aims to develop an alternative, ...

RNA-based drug susceptibility testing of Mycobacterium tuberculosis

is incubated in absence of the drug. Multiplex mRNA quantification is performed directly on crude cell lysates using a combination of the bead-based MagPixTM (Luminex) and QuantigeneTM Plex (Thermo ...

The AMR-ARRAY: A modular bead array detecting β-lactam, (fluoro) quinolone, colistin, aminoglycoside and macrolide resistance determinants in Gram-negative bacteria.

a 53-plex assay designed to target selected genetic markers with 3 internal controls. The AMR- ARRAY consistently detected resistance determinants as compared to phenotypically expressed resistance for 94.7% ...

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