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Targeted Whole Genome Sequencing of the Capripoxvirus Genome from Clinical Tissue Samples and Lyophilized Vaccine Batches.
of tiled amplicons. The resulting amplicons can be sequenced on all currently available high-throughput sequencing platforms. As an example, we have included a detailed protocol for performing nanopore ...
Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms
propose a targeted high-throughput sequencing approach, including (i) a prior PCR-based enrichment step to amplify regions of interest, (ii) a sequencing step, and (iii) a data analysis methodology to ...
Metagenomic Characterization of Multiple Genetically Modified Bacillus Contaminations in Commercial Microbial Fermentation Products
high-throughput sequencing to confirm and extend the results of classical analysis methods for the genomic characterization of unauthorized GMM. By combining short- and long-read metagenomic sequencing, two ...
Detection and identification of authorized and unauthorized GMOs using high-throughput sequencing with the support of a sequence-based GMO database
Publication Type: Peer reviewed scientific article Authors: Assia Saltykova; Julien Van Braekel; N. Papazova; Marie-Alice Fraiture; Deforce, Dieter; Kevin Vanneste; Sigrid C.J. De Keersmaecker; Nancy Roosens Source: Food Chemistry: Molecular Sciences, Vol ...
Key considerations for the implementation of high throughput sequencing based metagenomics (mNGS) in diagnostic clinical, food and veterinary labs: a no-nonsense pointer. the Metastava consortium
Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Steven Van Borm; Frank Vandenbussche; Kris De Clercq; Andy Haegeman; Sigrid C.J. De Keersmaecker; Kevin Vanneste; Michael Peeters; Steven Van Gucht; Yannick Blanchar ...
Evaluation of a commercial exogenous internal process control for diagnostic RNA virus metagenomics from different animal clinical samples
Virological Methods, Volume 283 (2020) Keywords: Animals Feces High-Throughput Nucleotide Sequencing Mengovirus Metagenomics Rna RNA Virus Infections RNA Viruses Swine Swine Diseases Viral Sensitivity and ...
Targeting the 16S rRNA gene for bacterial identification in complex mixed samples: comparative evaluation of second (Illumina) and third (Oxford Nanopore Technologies) generation sequencing technologies.
Keersmaecker; Kevin Vanneste Source: Int J Mol Sci, Volume 21, Issue 1 (2019) Keywords: bacteria DNA, Bacterial High-Throughput Nucleotide Sequencing Nanopores RNA, Ribosomal, 16S Abstract: Rapid, accurate ...
Bigger and Better? Representativeness of the Influenza A Surveillance Using One Consolidated Clinical Microbiology Laboratory Data Set as Compared to the Belgian Sentinel Network of Laboratories.
same period, as an added-value proposition. These results illustrate that the real-time integration of high-throughput whole genome sequencing platforms available in consolidated CML s into the public ...
Complete coding sequence of a novel picorna-like virus in a blackbird infected with Usutu virus.
Picornaviridae Infections Plants whole genome sequencing Abstract: Using random high-throughput RNA sequencing, the complete coding sequence of a novel picorna-like virus (a 9,228-nt contig containing 212,202 ...
Use of next‐generation sequencing in microbial risk assessment
High‐throughput tools such as next‐generation sequencing (NGS) could fill this gap. NGS approaches can be used to generate ample qualitative and quantitative data to be used in the risk assessment process. NGS ...