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Metagenomic Characterization of Multiple Genetically Modified Bacillus Contaminations in Commercial Microbial Fermentation Products
12, Issue 12 (2022) Keywords: Bacillus food enzyme genetically modified microorganisms (GMM) hybrid genome assembly metagenomic shotgun sequencing Abstract: Genetically modified microorganisms (GMM) are ...
Development of an NGS-Based Workflow for Improved Monitoring of Circulating Plasmids in Support of Risk Assessment of Antimicrobial Resistance Gene Dissemination.
low-copy-carrying plasmid (>200 kb) was used. Hybrid assemblies of NGS data obtained from whole-genome DNA extractions of the original isolates resulted in the most complete reconstruction of plasmids. The optimal ...