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EFSA Project on the use of New Approach Methodologies (NAMs) for the hazard assessment of nanofibres. Lot 1, nanocellulose oral exposure: gastrointestinal digestion, nanofibres uptake and local effects
Fessard, Valerie; Jan Mast; Blanquet‐Diot, Stephanie; Bremer‐Hoffmann, Susanne; Cubadda, Francesco Source: EFSA Supporting Publications, Volume 20, Issue 9 (2023) Keywords: barrier crossing cell uptake ...
RE-Place: A Unique Project Collecting Expertise on New Approach Methodologies
computer modeling, high throughput testing, omics, and sophisticated cell cultures, the use of experimental animals in the life sciences can be reduced or sometimes even completely avoided. Stimulating NAM ...
Characterization of two recombinant HVT-IBD vaccines by VP2 insert detection and cell-mediated immunity after vaccination of specific pathogen-free chickens.
objectives of this study were twofold: (i) to develop in vitro assays and molecular tools to detect the VP2 protein and gene and (ii) to evaluate cell-mediated immunity (CMI) induced by rHVT- IBD vaccination ...
Introducing speciation changes, human bioaccessibility and bioavailability in the risk evaluation of dietary inorganic arsenic intake: a Belgian case study
food matrices were obtained by means of the Caco-2 cell system. From these data, human intestinal absorption values were derived (bioavailability). To perform As in- and uptake calculations, different ...
Heterozygous inactivation of plasma membrane Ca(2+)-ATPase in mice increases glucose-induced insulin release and beta cell proliferation, mass and viability.
cell function. METHODS: Biological and morphological methods (Ca(2+) imaging, Ca(2+) uptake, glucose metabolism, insulin release and immunohistochemistry) were used to assess beta cell function and proliferation ...
HIV-1 IN/Pol recruits LEDGF/p75 into viral particles.
besides acting as a molecular tether in the target cell, also affects late steps of HIV replication. RESULTS: LEDGF /p75 is recruited into HIV-1 particles through direct interaction with the viral IN (or ...
Biological evaluation of bisbenzaldehydes against four Mycobacterium species.
Vanden Eynde, Jean Jacques; Huygen, Kris Source: Eur J Med Chem, Volume 63, p.731-8 (2013) Keywords: Animals Anti-Bacterial Agents Antitubercular Agents Benzaldehydes Cell Line Cell Survival Cells ...
Heterozygous inactivation of the Na/Ca exchanger increases glucose-induced insulin release, β-cell proliferation, and mass.
NCX1. RESEARCH DESIGN AND METHODS: Biologic and morphologic methods (Ca(2+) imaging, Ca(2+) uptake, glucose metabolism, insulin release, and point counting morphometry) were used to assess β-cell function ...
Membrane permeabilization and cellular death of Escherichia coli, Listeria monocytogenes and Saccharomyces cerevisiae as induced by high pressure carbon dioxide treatment.
treatment time (0-60 min) was examined. Loss of membrane integrity was measured as increased uptake of the fluorescent dye propidium iodide (PI) with spectrofluorometry, while cell inactivation was determined ...
Virulence-associated traits in avian Escherichia coli: comparison between isolates from colibacillosis-affected and clinically healthy layer flocks.
caecal and 20 extra-intestinal isolates. The examined characteristics were adhesins, invasivity in T84 cell culture, serum resistance, iron uptake, colicin production, and toxinogenicity. The following ...